Cholinesterase

Labkit

Butyrylthiocholine | kinetic

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Cholinesterases (ChEs) are a ubiquitous group of enzymes that hydrolyze esters of choline. A well-known example is acetylcholinesterase (AChE, acetylcholine hydrolase, EC 3.1.1.7), the enzyme responsible for hydrolyzing the important neurotransmitter acetylcholine (ACh). Another ChE is butyrylcholinesterase (BuChE, acylcholine acylhydrolase, EC 3.1.1.8), also known as nonspecific cholinesterase.The difference between them is that the acetylcholinesterase catalyzes the hydrolysis of acetylcholine in the muscles, nerves etc. while pseudocholinesterase is a plasma enzyme, produced by the liver and it hydrolyses butyrylcholine and a variety of choline esters.

 

In acute hepatitis, of infective or toxic origin, plasma cholinesterase activity falls modestly within a few days of onset, returning gradually to normal with recovery. Chronic hepatitis, cirrhosis, neoplastic and other infiltrative diseases of the liver also result in low activity. In obstructive jaundice, normal values are the rule, unless there is concomitant liver disease or the obstruction is due to malignancy, when reduced values are found. Malignant disease, even when localized and not involving the liver, can give low enzyme activity due to impaired enzyme synthesis. With fatty liver, levels are normal or increased. Cholinesterase is best studied serially and is of greatest value as a prognostic tool. A sudden or marked fall to a quarter of the usual activity indicates ominous deterioration. A large number of drugs have been reported as causing a reduction in Cholinesterase activity.

 

Assay of butyrylcholinesterase activity in plasma can be used as a liver function test and is a sensitive test to find exposure to pesticides organophosphorus and identification of patients with the atypical form of enzyme who presents high sensitivity to succinyl-choline. Both hypercholinesterasemia and hypocholinesterasemia indicate pathological processes. The half-life of BCHE is approximately 10 to 14 days.

Product Features:

  • Kinetic / butyrylthiocholine substrate for pseudocholinesterase assay.
  • Lyophilised reagent for longer open vial stability.
  • Working reagent stability 2 hours at 2-8°C.
  • Factor based calculation.
  • Controls are available and they are optional.
  • Ideal for usage on semiautomatic analyzers.
  • AMR = 7 ~ 9084 iU/L.

Product Code

Description

Pack Size

Standard/Control

 

 

30190

Generic pack

20 x 3mL.

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