Citric acid

Labkit

Citrate lyase | Colorimentric.

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Accumulation and secretion of extraordinarily high levels of citrate are principal functions of the prostate gland of humans and other animals. The function of citric acid in mammalian semen is not known with certainty. In man it appears in the human ejaculate in widely varying concentrations: 0-2340 mg/dL. Citric acid in semen has been regarded not as an energy source for spermatozoa, but as a component of semen having its primary function in the extracellular environment; jellification and liquefaction of seminal plasma, binding of calcium ions and interaction with hyaluronidase. Citricolysis in semen proceeds very slowly and does not serve to maintain respiratory rates in spermatozoa. On the other hand, active citricolysis requires the presence of sperm cells, and seminal plasma alone does not metabolize citric acid.

 

Concentration of citric acid can decrease, under the normal level, due to metabolic consumption in urine with bacterial contamination. Likewise, its decrease has been referred as responsible of the formation of kidney stone due to the chelating capacity of calcium. Level of citric acid can be elevated due to a diet rich in carbohydrates, oestrogens therapy, hepatic failure, metabolic acidosis, hyperparathyroidism and Vitamin D intoxication. It is one of the prostatic markers in seminal plasma. Furthermore, citrate metabolism is markedly altered in benign prostatic hyperplasia (BPH) and in prostatic carcinoma (CA).

Product features.

  • For seminal fluid and urine estimation of citric acid.
  • Enzymatic – colorimetric method.
  • Suitable for use on of semiautomatic analyzers.
  • Working reagent stability = 24 hours at 2~8°C / 30 days a -20°C.
  • Inclusive of citric acid standard.
  • AMR up to 400mg/L.
  • CE marked.

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