PCR-Covid 19 (RdRP & E gene)

Molecular biology Kits

One-step Multiplex Real-Time RT-PCR.

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Coronavirusesare a large family of viruses (Coronaviridae) that are known to cause illness ranging from the common cold to more severe diseases such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS). At least six species of coronaviruses have been found to cause diseases in humans. Common signs of these infections include respiratory symptoms, fever, cough, shortness of breath or other breathing difficulties etc. In more severe cases the infection can cause pneumonia, SARS, kidney failure and even be fatal. Novel coronavirus pneumonia is caused by novel coronavirus (SARS CoV 2). WHO has officially named after the disease Covid-19, signifying the year of outbreak?

 

Viral tests are recommended to diagnose acute infection. Authorized assays for viral testing include those that detect SARS-CoV-2 nucleic acid or antigen. Q-Line® Molecular (nCoV-19) RT-PCR detection kit (E & RdRP gene) is designed to detect nCoV-19 based on single tube multiplex rRT-PCR assays in respiratory and serum specimens. The high quality primers and probes specific for nCoV-19: E-gene and RdRP offers a high sensitivity and specificity.With the PCR reaction mix provided, the amplification of template are quantitatively monitored by the increased fluorescence signal detected by a RT-PCRinstrument.The PCR detection system includes human housekeeping gene as IPC, primer and probe mix.

 

The test kit is complemented with validated nucleic acid extraction products best suited for individual laboratory need. Also refer to the below products:

PRODUCT FEATURES:

  • One tube reaction for identification and detection of 2019-nCoV (E-gene & RdRP)
  • One-step Multiplex Real-Time RT-PCR.
  • Human housekeeping gene as IPC to ensure a potential false negative value due to non-specific PCR inhibition of a sample.
  • Limit of detection (LOD): 100 copies/mL.
  • Reliable and reproducible results.
  • No cross reactivity with other respiratory viruses.
  • Sensitivity : 98.7%
  • Specificity : 100%
  • Compatible with ABI 7500/7500 Fast, Qiagen Rotor Q5, CFX96, Roche Light Cycler 480 and many other systems with FAM, HEX/VIC, RED/ROX, CY5 Channels.
  • 1 year shelf life at -20°C

Product Code

Description

Units

Additional

 

 

nCoV-19ER100PS

100 Tests

100 Tests

Yes

Optional

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