LDH - (Lactate dehydrogenase)

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LDH - (Lactate dehydrogenase)

Product Description

Pyruvate to lactate | DGKC | Kinetic

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LDH is an intracellular enzyme which is widely distributed throughout the body and is found at high levels in tissues that utilize glucose for energy; it is therefore not organ specific. Structurally, the enzyme is a tetramer with the two most common protein subunits LDH-M (M=muscle) and LDH-H (H=heart), in differentcombinations forming five isoenzymes: H4, M4, and the three mixed tetramers (H3M1, H2M2, H1M3). Though theisoforms are enzymatically similar in function,their distribution varies with different tissues:

 

LDH-1 (H4) is found in the heart and in RBC (red blood cells), as well as the brain.

LDH-2 (H3M1) is in the reticuloendothelial system.

LDH-3 (H2M2) is found in the lungs.

LDH-4 (H1M3) is found in the kidneysplacenta, and pancreas.

LDH-5 (M4) is found in the liver and striated muscle, also present in the brain.

 

LDH catalyzes the conversion of pyruvate to lactate with the regeneration of NADH to NAD+. This conversion is essential in hypoxic and anaerobic conditions when ATP production by oxidative phosphorylation is disrupted. As a consequence, LDH activity is upregulated in those conditions. Since the enzyme is widely distributed, an increase in LDH can reflect damage to a number of different tissues but not one in specific unless isozymes were quantified. LDH levels may be increased whenever there is cell necrosis or when neoplastic proliferation of cells causes an increase LDH production.

Product Features:

  • Pyruvate to lactate conversion / DGKC.
  • Kinetic rate, UV method.
  • Both ready to use reagents as well as lyophilized reagents for different customers’ choice.
  • Wide AMR: ranging between 5.5 and 1453 iU/L and AMR =3.42 ~ 1700 iU/L for kits 30293s, 30293 and 30294.
  • A good correlation with other brands as well as DGKC-SCE method.
  • Longer working reagent stability ? 5 days at 2-8°C. / 24hours at R.T, making it ideal for semiautomatic instrument users.
  • Longer working reagent stability ?15 days at 2-8°C. / 5 days at R.T, making it more suitable for semiautomatic instrument users with very less workload (prdt. Code 30293/s, 30293 and 30294)
  • Programmable on most of the fully automated biochemistry analyzers

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