Product Description

Vitamin-D3 25-OH

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Vitamin D is well known for its classic role in the maintenance of bone mineral density. In conjunction with parathyroid hormone (PTH), Vitamin D plays a critical role in calcium homeostasis. However, in recent years, Vitamin D has been receiving increased attention due to the resurgence of Vitamin D deficiency and rickets in developed countries. High prevalence of vitamin D deficiency is also seen throughout India. Many studies on the incidence of Vitamin D deficiency (i.e., 25-(OH) D< 20 ng/mL) in community-based Indian studies on apparently healthy controls reported a prevalence ranging from 50% to 94% for a last one decade. Numerous scientific and medical researchers have provided strong evidence supporting the correlations between Vitamin D insufficiency and increased risks of developing non-skeletal pathologies including cancer, heart diseases, stroke, diabetes, hypertension, autoimmune diseases, infectious diseases, and aging.

In the blood, Vitamin D exists as a protein bound form, specifically with a Vitamin D binding protein. Clinically, Vitamin D levels in the blood get reported as the total 25-OH Vitamin D, which is the sum of 25-(OH) D3 and 25-(OH) D2.Traditionally, analytical methods for Vitamin D determination have been measured by competitive binding, high-performance liquid chromatography (HPLC), and radioimmunoassay (RIA) methods. However, despite their functionality, these methods (HPLC, RIA, and LC-MS/MS) tend to be labour intensive and technically difficult. They are often not fully automated, have low throughputs, and LC-MS/MS method is not commonly available in most clinical laboratories.

The innovative Q-Line Platinum Vitamin D assay is a 2-reagent, direct particle enhanced immunoturbidimetric assay for use on general clinical chemistry analyzers, without need of any cumbersome sample preparatory steps or special instrument.



  • Measure Vitamin D2 & D3 equally
  • Latex Enhanced immunoturbidimetric Assay
  • Two Vials liquid stable reagents
  • Convenient Pack Size - 25 mL (R1: 20 mL + R2 : 5 mL)
  • Fastest results in 10 minutes
  • No risk of Biotin Interference
  • No sample pre - treatment, low sample volume required
  • Wide Linearity Range from 7.6 - 147.8 ng/mL
  • Multipoint Calibration & Two-Level Controls liquid stable
  • Excellent Assay Precision of CV % < 5%, good sensitivity
  • Excellent correlation to LC-MS/MS method
  • Wide range of instrument applications available.


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